CMT - How To Use

CMT SINGLE Test
Using scissors carefully cut out the required number of single test vials. Pierce or remove the foil covering each test vial to allow entry of the sample pipet.
Using the special No Drop Count Pipet included in the kit add 100µl of milk sample into each test vial. Ensure that a new clean pipet is used for each sample. Refer to the pack insert for specific instructions on how to use the No Drop Count Pipets.
Incubate the test vials in special heated incubation block or in a water bath heated to 64°C +/- 1°C. For incubation in a water bath Copan supply unique foam floating racks to hold the test vials. It is recommended that when incubating in a water bath that the test vials are properly covered to avoid problems of water condensation. Incubate the tests for 3 hrs.
A color chart is provided as a guide for interpretation of positive and negative color reactions. Read the color and score the result of each test by examining the bottom and sides of the vials.

CMT MICROPLATE
The MICROPLATE has 96 test wells divided into 6 strips of 16. As few as 16 tests can be performed at any one time. The operator can separate a strip of 16 wells from the MICROPLATE by simply breaking away a strip. Hold the MICROPLATE so the bottom of the wells is facing upwards and the foil cover is down. Bend the plate along a line between a row of 16 wells to break and release the strip, then carefully use scissors to cut the aluminum foil covering to fully separate the strip from the rest of the MICROPLATE.
Using a volumetric micro pipetor add 100µl of milk sample into each test well. It is important to remember to use a new clean pipet tip for each sample. Take care to avoid splashes, overspill and cross contamination between adjacent wells. Seal the well of the MICROPLATE strip using the adhesive film provided in the kit.
Incubate the MICROPLATE or strips of the plate in a heated incubation block or water bath at 64°C +/- 1°C. It is essential that when incubating in a water bath that the MICROPLATE strips must be covered with adhesive film to avoid problems of water condensation. Incubate the tests for 3 hrs.
A color chart is provided as a guide for interpretation of positive and negative color reactions. Read the color and score the result of each test by examining the bottom MICROPLATE wells.